Other

Part:BBa_I716307:Design

Designed by: Vincent Parker   Group: iGEM07_Berkeley_UC   (2007-10-25)


OtsBA-TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Digest Bca1144-I716303 (EcoRI, BamHI 2215bp, 2063bp. L) Sub into pBca9145-Bca1092 (EcoRI, BglII 2198bp, 9bp. L) Product is I716307

Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References