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Other
Part:BBa_I716307:Design
Designed by: Vincent Parker Group: iGEM07_Berkeley_UC (2007-10-25)
OtsBA-TT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2351 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Digest Bca1144-I716303 (EcoRI, BamHI 2215bp, 2063bp. L) Sub into pBca9145-Bca1092 (EcoRI, BglII 2198bp, 9bp. L) Product is I716307
Source
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.